[1]谭光辉,李杰章,覃媛钰,等.三穗鸭ORAI1基因遗传变异与蛋壳品质的相关性[J].福建农林大学学报(自然科学版),2020,49(05):646-651.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2020.05.011]
 TAN Guanghui,LI Jiezhang,QIN Yuanyu,et al.Association analysis of ORAI1 genetic variation and eggshell quality in Sansui ducks[J].,2020,49(05):646-651.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2020.05.011]
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三穗鸭ORAI1基因遗传变异与蛋壳品质的相关性()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
49卷
期数:
2020年05期
页码:
646-651
栏目:
动物科学
出版日期:
2020-09-18

文章信息/Info

Title:
Association analysis of ORAI1 genetic variation and eggshell quality in Sansui ducks
文章编号:
1671-5470(2020)05-646-06
作者:
谭光辉12 李杰章12 覃媛钰12 吴 磊12 张依裕12
1.贵州大学动物科学学院; 2.贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵州 贵阳 550025
Author(s):
TAN Guanghui12 LI Jiezhang12 QIN Yuanyu12 WU Lei12 ZHANG Yiyu12
1.College of Animal Science, Guizhou University; 2.Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education,Guizhou University, Guiyang, Guizhou 550025, China
关键词:
三穗鸭 ORAI1基因 遗传变异 相关性
Keywords:
Sansui duck ORAI1 gene genetic variation correlation
分类号:
S834.2
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2020.05.011
文献标志码:
A
摘要:
在NCBI GenBank数据库中获取鸭ORAI1基因组序列,采用Primer 3.0程序设计引物扩增该基因全部编码区,PCR扩增产物纯化后直接测序,采用DNAStar软件中的MegaAlign程序结合测序结果筛查鉴定单核苷酸突变(SNP)位点,并运用SPSS 18.0软件分析SNP位点与蛋壳品质的相关性,旨在探究三穗鸭钙释放激活钙调节蛋白1基因的遗传变异及其与蛋壳品质的相关性,为改善蛋壳品质提供新的科学资料.结果表明,只在三穗鸭ORAI1基因第3外显子发现3个同义突变位点,分别是g.16789 C>T、g.16984 C>T和g.17074 A>G.相关性分析显示:g.16789 C>T、g.16984 C>T和g.17074 A>G位点对蛋壳强度的影响达到显著水平(P<0.05),其中,g.16789 C>T和g.16984 C>T两个位点CT杂合基因型个体的蛋壳强度显著高于TT纯合基因型,g.17074 A>G位点GG基因型个体的蛋壳强度显著高于AG基因型(P<0.05),揭示CT和GG是有利基因型; 3个SNP位点组合基因型对蛋壳强度和厚度均有不同程度的影响,其中,H2H3和H3H3双倍型个体的蛋壳厚度显著高于H2H2型(P<0.05),H3H3型的蛋壳强度显著高于H2H2型(P<0.05).揭示在ORAI1基因第3外显子检测到的3个SNP位点与蛋壳品质具有相关性,可以作为改善鸭蛋壳品质分子标记选育的资料.
Abstract:
To investigate the genetic variation of ORAI1, whose product ORAI1 is a subunit of the calcium release-activated calcium modulator 1 channel and its association with eggshell quality in Sansui duck, the sequence of the ORAI1 gene was obtained from NCBI GenBank, then the entire coding regions of the ORAI1 gene were amplified by the primers designed via Primer 3.0. After amplification and purification, single nucleotide polymorphism(SNP)were screened and identified by the MegaAlign program in DNAStar software combined with sequencing peak map. The genetic effects of SNPs loci on eggshell quality were further analyzed by SPSS 18.0 general linear model. Three synonymous mutation sites, g.16789 C>T, g.16984 C>T and g.17074 A>G, were detected in the exon-3 of the ORAI1 gene. Correlation analysis revealed that all 3 loci exerted significant effects on eggshell strength(P<0.05), among which the eggshell strength of heterozygous CT genotype at loci of g.16789 C>T and g.16984 C>T was significantly higher than that of TT genotype, and that of homozygous GG at the g.17074 A>G locus was significantly higher than that of heterozygous AG(P<0.05), indicating that CT and GG were favorable genotypes. The combined genotypes of the 3 SNP had different effects on eggshell thickness and strength, among which the eggshell thickness of H2H3 and H3H3 individuals were significantly higher than that of H2H2(P<0.05)and shell strength of H3H3 was significantly higher than that of H2H2(P<0.05). The comprehension of the correlations between 3 single nucleotide mutations detected in the exon-3 of the ORAI1 gene and eggshell quality is attributed to the knowledge of molecular marker and breeding for eggshell with better quality.

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备注/Memo

备注/Memo:
收稿日期:2019-08-09 修回日期:2019-10-31
基金项目:国家自然科学基金资助项目(31760663); 贵州省科技人才补助项目(黔科合平台人才[2017]5788); 贵州省千层次创新人才项目(701030174401).
作者简介:谭光辉(1996-),男.研究方向:动物遗传育种与繁殖.Email:1207080679@qq.com.通信作者张依裕(1976-),男,副教授,硕士生导师,博士.研究方向:动物遗传资源保护与利用.Email: zyy8yyc@163.com.
更新日期/Last Update: 2020-09-20