[1]张以宏.泉州中华蜜蜂遗传多样性分析[J].福建农林大学学报(自然科学版),2020,49(05):671-677.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2020.05.015]
 ZHANG Yihong.Genetic diversity of Apis cerana from Quanzhou City, Fujian Province[J].,2020,49(05):671-677.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2020.05.015]
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泉州中华蜜蜂遗传多样性分析()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
49卷
期数:
2020年05期
页码:
671-677
栏目:
动物科学
出版日期:
2020-09-18

文章信息/Info

Title:
Genetic diversity of Apis cerana from Quanzhou City, Fujian Province
文章编号:
1671-5470(2020)05-0671-07
作者:
张以宏
泉州市畜牧站,福建 泉州 362000
Author(s):
ZHANG Yihong
Quanzhou Animal Husbandry Station, Quanzhou, Fujian 362000, China
关键词:
中华蜜蜂 遗传特征 形态标记 微卫星标记 tRNAleu~COII
Keywords:
Apis cerana genetic characteristics morphological marker SSR markers tRNAleu~COII.
分类号:
S891
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2020.05.015
文献标志码:
A
摘要:
采用33个形态标记、38个微卫星标记和tRNAleu~COII片段的线粒体标记,对泉州7个县市,共487只中华蜜蜂进行遗传分化、遗传特征和遗传多样性分析.研究结果表明,泉州样点均属于同一种群,无遗传分化.线粒体tRNAleu~COII片段共检测到29种单倍型,其中9种为新发现单倍型; 泉州中华蜜蜂处于中度多样性水平,期望杂合度(He)在0.440 7~0.513 1之间,观察杂合度(Ho)在0.406 2~0.512 4之间,平均多态信息含量(PIC)在0.409 1~0.476 5之间,单倍型多样性(Hd)的范围0.241~0.739,平均核苷酸差异数(K)为0.339~0.926,核苷酸多样度(Pi)为0.000 96~0.003 34.
Abstract:
To study the genetic diversity and geographical differentiation of Apis cerana habitating in Quanzhou City, southeast China's Fujian Province. 497 samples of A.cerana from 7 counties were analyzed using 33 morphological markers, 38 microsatellite markers and mitochondrial marker of tRNAleu~COII fragments. The results showed that A.cerana from Quanzhou do not genetically differentiate and all belong to 1 population. A total of 29 haplotypes were detected based on tRNAleu~COII fragments, of which 9 were newly discovered haplotypes. This honeybee population stays in moderate diversity level, with the expected heterozygosity(He)ranging from 0.440 7 to 0.513 1 and heterozygosity observed(Ho)ranging from 0.406 2 to 0.512 4. Polymorphisms information content averaged between 0.409 1 and 0.476 5, and haplotype diversity(Hd)varied between 0.241 and 0.739. Average nucleotide difference(K)ranged from 0.339 to 0.926, resulting in a nucleotide diversity of 0.000 96 to 0.003 34.

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备注/Memo

备注/Memo:
收稿日期:2020-02-22 修回日期:2020-03-15
作者简介:张以宏(1968-),男,高级畜牧师.研究方向:畜禽遗传资源保护.Email:bee8788@sohu.com.
更新日期/Last Update: 2020-09-20