[1]范晓月,何云核,赵富荣,等.喜树碱与10-羟基喜树碱对5种植物的化感作用[J].福建农林大学学报(自然科学版),2020,49(05):703-711.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2020.05.019]
 FAN Xiaoyue,HE Yunhe,ZHAO Furong,et al.Allelopathic effects of camptothecin and 10-hydroxycamptothecin on five plants[J].,2020,49(05):703-711.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2020.05.019]
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喜树碱与10-羟基喜树碱对5种植物的化感作用()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
49卷
期数:
2020年05期
页码:
703-711
栏目:
资源与环境
出版日期:
2020-09-18

文章信息/Info

Title:
Allelopathic effects of camptothecin and 10-hydroxycamptothecin on five plants
文章编号:
1671-5470(2020)05-0703-09
作者:
范晓月1 何云核1 赵富荣2 郭 明13
1.浙江农林大学风景园林与建筑学院; 2.林业与生物技术学院; 3.理学院, 浙江 杭州 311300
Author(s):
FAN Xiaoyue1 HE Yunhe1 ZHAO Furong2 GUO Ming13
1.School of Landscape Architecture; 2.School of Forestry and Bio-technology; 3.School of Science, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China
关键词:
喜树碱 10-羟基喜树碱 草本观赏植物 生态效益 化感作用
Keywords:
camptothecin 10-hydroxycamptothecin herbaceous ornamental plant ecological benefits allelopathic effect
分类号:
S459
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2020.05.019
文献标志码:
A
摘要:
采用培养皿萌发试验和盆栽试验,研究喜树碱与10-羟基喜树碱对白车轴草、大吴风草、三色堇、麦冬和鸢尾的作用.通过检测5种草本观赏植物的萌发率、萌发指数、叶片相对电导率、叶绿素含量、过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、脯氨酸和可溶性蛋白等生长和生理指标,考察它们对化感物质喜树碱与10-羟基喜树碱的耐受性.结果表明,5种植物受到不同程度的化感抑制作用,其中,综合效应指数分析显示5种植物化感作用的敏感性趋势为:白车轴草>三色堇>鸢尾>大吴风草>麦冬; 化感胁迫下5种植物的相对电导率、MDA和脯氨酸含量随着化感物质含量的增加均呈上升趋势; 叶绿素含量基本表现为抑制作用; POD、SOD、CAT活性呈先升后降趋势,在浓度为10 mg·L-1时达到最大; 浓度为20 mg·L-1时,5种植物的可溶性蛋白达到最大.
Abstract:
Germination test and pot experiment were carried out to study the effects of alkaloid from Camptotheca acuminate, camptothecin(CPT)and 10-hydroxycamptothecin(HCPT)on the growth of Trifolium repens, Farfugium japonicum, Viola tricolor, Ophiopogon japonicus and Iris tectorum. Tolerances to allelochemicals were evaluated based on growth indexes of seed germination rate and germination index, and physiological indexes of leaf relative conductivity, chlorophyll content, peroxidase(POD), superoxide dismutase(SOD), catalase(CAT), malondialdehyde(MDA), proline(Pro)and soluble protein(SP). The results showed that all 5 herbs were inhibited by the allelopathic effects to some extents. Analysis based on the comprehensive effect index showed that the sensitivity of the herbs to the allelopathic effects of CPT and HCPT followed the order of T.repens>V.tricolo>I.tectorum>F.japonicum>O.japonicus. The relative conductivity, MDA and Pro contents of the 5 plants showed upward trends under the allelopathic stress. Chlorophyll contents were mostly inhibited. POD, SOD, CAT contents increased first and then decreased, plateauing at a concentration of 10 mg·L-1 CPT and HCPT mixture. SP level reached its maximum at a concentration of 20 mg·L-1.

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备注/Memo

备注/Memo:
收稿日期:2020-01-21 修回日期:2020-03-20
基金项目:国家自然科学基金(31470587); 浙江省省院合作科技项目(2015SY11).
作者简介:范晓月(1995-),女.研究方向:野生园林植物资源分类与应用.Email:m15858237607_1@163.com.通信作者何云核(1960-),教授.研究方向:野生园林植物资源分类与应用.Email:lab406@sina.cn.
更新日期/Last Update: 2020-09-20