[1]章淑玲,欧高政,陈美玲.甘薯根结线虫病病原——象耳豆根结线虫的鉴定[J].福建农林大学学报(自然科学版),2021,50(01):23-28.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.01.004]
 ZHANG Shuling,OU Gaozheng,CHEN Meiling.Identification of root-knot nematode Meloidogyne enterolobii on sweet potato[J].,2021,50(01):23-28.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.01.004]
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甘薯根结线虫病病原——象耳豆根结线虫的鉴定()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年01期
页码:
23-28
栏目:
植物保护
出版日期:
2021-01-18

文章信息/Info

Title:
Identification of root-knot nematode Meloidogyne enterolobii on sweet potato
文章编号:
1671-5470(2021)01-0023-06
作者:
章淑玲1 欧高政1 陈美玲2
1.福建农业职业技术学院园艺园林学院,福建 福州 350119; 2.南安市天禾绿保农资有限公司,福建 泉州 362300
Author(s):
ZHANG Shuling1 OU Gaozheng1 CHEN Meiling2
1.College of Horticulture and Garden, Fujian Vocational College of Agriculture, Fuzhou, Fujian 350119, China; 2.Nan'an Tianhe Green Agricultural Means of Production Co. Ltd., Quanzhou, Fujian 362300, China
关键词:
象耳豆根结线虫 甘薯 福建省 形态鉴定 特异性PCR检测
Keywords:
Meloidogyne enterolobii sweet potato Fujian Province morphological identification specific PCR detection
分类号:
S432.4
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.01.004
文献标志码:
A
摘要:
自福建省泉州市发生严重根结线虫病的甘薯地采集线虫样本,经形态学观察发现该线虫与象耳豆根结线虫(Meloidogyne enterolobii)原始描述相符; rDNA-ITS序列分析表明,其与GenBank中象耳豆根结线虫同区域序列的相似性达99%以上; 利用象耳豆根结线虫特异性引物进行PCR扩增,得到236 bp的特异性片段,据此确定所采集的甘薯根结线虫为象耳豆根结线虫.这是首次在福建省甘薯上发现象耳豆根结线虫.
Abstract:
Nematode samples were collected from a sweet potato field with severe root-knot nematode disease in Quanzhou, Fujian Province, China. The morphological characteristics of the samples were consistent with the original descriptions of Meloidogyne enterolobii. The sequences of rDNA-ITS shared a high degree of genetic similarity(>99%)with the relevant regional sequences of M.enterolobii. The specific fragment of 236 bp was obtained by PCR amplification using species-specific primers of M.enterolobii. Based on the above results, the species of root-knot nematode on sweet potato was identified as M.enterolobii. This is the first report of M.enterolobii on sweet potato in Fujian Province.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2020-05-04 修回日期:2020-07-01
基金项目:国家自然科学基金项目(30370931); 福建农业职业技术学院重点课题(2019JS014).
作者简介:章淑玲(1978-),女,副教授,博士.研究方向:植物检疫和植物线虫学.Email:41524629@qq.com.
更新日期/Last Update: 2021-01-15