[1]刘 嘎,蒙小刚,史量全,等.一株牛源黄腐短杆菌的分离鉴定[J].福建农林大学学报(自然科学版),2021,50(01):103-107.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.01.014]
　LIU Ga,MENG Xiaogang,SHI Liangquan,et al.Isolation and identification of a Brevibacterium luteolum strain from calf[J].,2021,50(01):103-107.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.01.014]
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一株牛源黄腐短杆菌的分离鉴定()
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福建农林大学学报（自然科学版）[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年01期
页码:
103-107
栏目:
动物科学
出版日期:
2021-01-18

文章信息/Info

Title:
Isolation and identification of a Brevibacterium luteolum strain from calf
文章编号:
1671-5470(2021)01-0103-05
作者:
刘 嘎1;  蒙小刚1; 2;  史量全1; 2;  东彦新1;  潮洛蒙1;  刘 锴1; 2;  徐 静1
1.内蒙古民族大学动物科技学院; 2.内蒙古自治区肉牛疾病防控工程技术研究中心,内蒙古 通辽 028000
Author(s):
LIU Ga1;  MENG Xiaogang1; 2;  SHI Liangquan1; 2;  DONG Yanxin1;  CHAO Luomeng1;  LIU Kai1; 2;  XU Jing1
Research Center for Beef Disease Prevention and Control Engineering, Tongliao, Inner Mongolia 028000, China
关键词:
牛;  黄腐短杆菌;  鉴定;  PCR;  遗传进化树;  药物敏感性
Keywords:
cattle;  Brevibacterium luteolum;  identification;  PCR;  genetic evolution tree;  drug sensitivity
分类号:
S852.6
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.01.014
文献标志码:
A
摘要:
从犊牛腐烂的牛蹄上分离病原菌,通过菌株培养、BD phoenixTM 100生化鉴定、致病性试验、遗传进化树分析等方法,对该菌进行鉴定.结果表明,所分离菌株16S rRNA基因序列与GenBank已经上传的黄腐短杆菌同源性达99%,并最终确定该病原菌为黄腐短杆菌(Brevibacterium luteolum),编号为LG-01.药敏试验结果显示,该病原菌对对氟苯尼考、庆大霉素、氧氟沙星等9种抗生素敏感,对卡那霉素、丁胺卡那、红霉素产生了耐药性.
Abstract:
A pathogenic bacterium from the calf rotten hoof, named strain LG-01, was isolated and identified through bacterial culture, BD phoenixTM 100 biochemical identification, pathogenicity test, and genetic evolution tree analysis. The results showed that the 16S rRNA sequence of strain LG-01 shared 99% homology with Brevibacterium luteolum according to GenBank. Drug susceptibility results showed that the pathogen was sensitive to 9 antibiotics including florfenicol, gentamicin and ofloxacin, while it was resistant to kanamycin, amikacin and erythromycin.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2020-04-23 修回日期:2020-05-26
基金项目:内蒙古自治区高等学校科学研究项目(NJZY20111).
作者简介:刘嘎(1998-),男,研究方向:动物疾病的诊断.Email:1325289800@qq.com.通信作者徐静(1981-),女,讲师,博士.研究方向:人兽共患病病原的诊断及预防.Email:xujing.1234@163.com.
更新日期/Last Update: 2021-01-15