[1]程 敏,顾 钢,肖 顺,等.芽孢杆菌(Bacillus)FJSC01对烟草土传病原菌的拮抗作用及其鉴定[J].福建农林大学学报(自然科学版),2021,50(02):164-169.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.02.003]
　CHENG Min,GU Gang,XIAO Shun,et al.Antagonistic effects of Bacillus FJSC01 on soilborne pathogens of tobacco and its identification[J].,2021,50(02):164-169.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.02.003]
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芽孢杆菌(Bacillus)FJSC01对烟草土传病原菌的拮抗作用及其鉴定()
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福建农林大学学报（自然科学版）[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年02期
页码:
164-169
栏目:
植物保护
出版日期:
2021-02-15

文章信息/Info

Title:
Antagonistic effects of Bacillus FJSC01 on soilborne pathogens of tobacco and its identification
文章编号:
1671-5470(2021)02-0164-06
作者:
程 敏1;  顾 钢2;  肖 顺1;  周 挺2;  刘国坤1
1.福建农林大学植物保护学院,福建 福州 350002; 2.福建省烟草公司烟草科学研究所,福建 福州 350003
Author(s):
CHENG Min1;  GU Gang2;  XIAO Shun1;  ZHOU Ting2;  LIU Guokun1
1.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.Institute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou, Fujian 350003, China
关键词:
甲基营养型芽孢杆菌;  生物防治;  烟草;  土传病原
Keywords:
Bacillus methylotrophicus;  biological control;  tobacco;  soilborne pathogeny
分类号:
S476
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.02.003
文献标志码:
A
摘要:
从番茄根际土壤分离到1株芽孢杆菌(Bacillus)FJSC01,其对烟草土传病原菌青枯拉尔氏菌(Ralstonia solanacearum)、胡萝卜软腐果胶杆菌(Pectobacterium carotovorum)、尖镰孢菌(Fusarium oxysporum)、胶孢炭疽菌(Colletotrichum gloeosporioides)、立枯丝核菌(Rhizoctonia solani)、疫霉菌(Phytophthora parasitica)和灰葡萄孢菌(Botrytis cinerea)等具有明显的拮抗作用.盆栽试验结果表明,菌株FJSC01对烟草青枯病的防效为40%.基于病原菌培养性状、形态特征、生理生化特性、16S rDNA系统演化分析,将菌株FJSC01鉴定为甲基营养型芽孢杆菌(Bacillus methylotrophicus).
Abstract:
Bacillus FJSC01 was isolated from the rhizosphere of tomato. It showed a strong antagonistic effect on tobacco soilborne pathogens, including bacteria such as Pectobacterium carotovorum and Ralstonia solanacearum, fungi such as Botrytis cinerea, Colletotrichum gloeosporioides, Fusarium oxysporum and Rhizoctonia solani, and oomycete of Phytophthora parasitica. Pot experiment showed that the bio-control efficacy of Bacillus FJSC01 against tobacco bacterial wilt disease caused by R.solanacearum reached 40%. Based on the culture traits, morphological characteristics, physiological and biochemical characteristics, and 16S rDNA gene phylogenetic analysis, Bacillus FJSC01 was identified as Bacillus methylotrophicus.

参考文献/References:

[1] 陈利锋,徐敬友.农业植物病理学[M].3版.北京:中国农业出版社,2007.
[2] 张绍升,顾钢,刘长明.烟草病虫害诊治图鉴[M].福州:福建科学技术出版社,2012.
[3] GOUDA S, KERRY R G, DAS G, et al. Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture[J]. Microbiological Research, 2018,206:131-140.
[4] NIU D, LIU H, JIANG C, et al. The plant growth-promoting rhizobacterium Bacillus cereus AR156 induces systemic resistance in Arabidopsis thaliana by simultaneously activating salicylate and jasmonate[J]. Molecular Plant-Microbe Interactions, 2011,24(5):533-542.
[5] MEENA M K, GUPTA S, DATTA S. Antifungal potential of PGPR, their growth promoting activity on seed germination and seedling growth of winter wheat and genetic variability among bacterial isolates[J]. International Journal of Clinical & Experimental Pathology, 2016,8(10):235-243.
[6] GROSCH R, JUNGE H, KREBS B, et al. Use of Bacillus subtilis as biocontrol agent. Ⅲ. Influence of Bacillus subtilis on fungal root diseases and on yield in soilless culture[J]. Journal of Plant Diseases and Protection, 1999,106:568-580.
[7] SHAFI J, TIAN H, JI M. Bacillus species as versatile weapons for plant pathogens: a review[J]. Agriculture and Environmental Biotechnology, 2017,31(3):446-459.
[8] BORRISS R. Bacillus, a plant beneficial bacterium, in principles of plant-microbe interactions[M]∥LUGTENBERG B. Microbes for sustainable agriculture. Berlin: Springer, 2015:379-391.
[9] FIRA D, DIMKIC I, BERIC T, et al. Biological control of plant pathogens by Bacillus species[J]. Journal of Biotechnology, 2018,285(10):44-55.
[10] 赵斌,何绍江.微生物学实验[M].北京:科学出版社,2002.
[11] 全国烟草标准化技术委员会.烟草病害分级及调查方法:YC/T 39—1996[S].北京:国家技术监督局,1996.
[12] 方中达.植病研究方法[M].北京:中国农业出版社,1998.
[13] 布坎南R E,吉本斯N E.伯杰细菌鉴定手册[M].8版.中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组,译.北京:科学出版社,1984.
[14] 陶天申,东秀珠.原核生物系统学[M].北京:化学工业出版社,2007.
[15] WEISBURG W G, BARNS S M, PLEEETIER D A, et al. 16S ribosomal DNA amplification for phylogenetic study[J]. Journal of Bacteriology, 1991,173(2):697-703.
[16] MILLER M, PFEIFFER W, SCHWARTZ T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees[C]∥Gateway Computing Environments Workshop. New York: IEEE, 2010:1-8.
[17] RONQUIST F, HUELSENBECK J P. MrBayes 3: Bayesian phylogenetic inference under mixed models[J]. Bioinformatics, 2010(19):1572-1574.
[18] MADHAIYAN M, POONGUZHALI S, LEE J S, et al. Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil[J]. Journal of Systematic & Evolutionary Microbiology, 2010,60(10):2490-2495.
[19] 冯云利,奚家勤,马莉,等.烤烟品种NC297内生细菌中拮抗烟草黑胫病的生防菌筛选及种群组成分析[J].云南大学学报(自然科学版),2011,33(4):488-496.
[20] 陈新春,张喜喜,汪钱龙,等.蔬菜根际细菌R2-2的鉴定及其抑菌活性[J].湖南农业大学学报(自然科学版),2012,38(2):177-180.
[21] 李祖红,曾嵘,陈龙,等.一株Bacillus methylotrophilus的鉴定及对烟草疫霉防效研究[J].云南农业大学学报(自然科学版),2014,29(6):933-936.
[22] 周登博,陈宇丰,井涛,等.抗香蕉枯萎病甲基营养型芽孢杆菌的鉴定及定殖[J].中国农学通报,2017,33(36):145-151.
[23] 王洪梅,吴成云,沈标.青枯病生防菌 N5的特性及其生物学效应[J].土壤,2013,45(6):1082-1090.
[24] 夏艳,徐茜,董瑜,等.烟草青枯病菌拮抗菌的筛选、鉴定及生防特性研究[J].中国生态农业学报,2014,22(2):201-207.
[25] 刘伟,刘鹏,沈小英,等.烟草青枯病拮抗芽孢杆菌的筛选、鉴定及其抑菌活性初探[J].西北农林科技大学学报(自然科学版),2014,42(2):123-130.

备注/Memo

备注/Memo:
收稿日期:2020-05-25 修回日期:2020-07-16
基金项目:中国烟草总公司福建省公司科技项目(201735000024112).
作者简介:程敏(1984-),女.研究方向:植物病害生防菌.Email:2453315920@qq.com.通信作者刘国坤(1972-),男,教授.研究方向:植物病害生物防治.Email:liuguok@126.com.
更新日期/Last Update: 2021-02-15