[1]程 敏,顾 钢,肖 顺,等.芽孢杆菌(Bacillus)FJSC01对烟草土传病原菌的拮抗作用及其鉴定[J].福建农林大学学报(自然科学版),2021,50(02):164-169.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.02.003]
 CHENG Min,GU Gang,XIAO Shun,et al.Antagonistic effects of Bacillus FJSC01 on soilborne pathogens of tobacco and its identification[J].,2021,50(02):164-169.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.02.003]
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芽孢杆菌(Bacillus)FJSC01对烟草土传病原菌的拮抗作用及其鉴定()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年02期
页码:
164-169
栏目:
植物保护
出版日期:
2021-02-15

文章信息/Info

Title:
Antagonistic effects of Bacillus FJSC01 on soilborne pathogens of tobacco and its identification
文章编号:
1671-5470(2021)02-0164-06
作者:
程 敏1 顾 钢2 肖 顺1 周 挺2 刘国坤1
1.福建农林大学植物保护学院,福建 福州 350002; 2.福建省烟草公司烟草科学研究所,福建 福州 350003
Author(s):
CHENG Min1 GU Gang2 XIAO Shun1 ZHOU Ting2 LIU Guokun1
1.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.Institute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou, Fujian 350003, China
关键词:
甲基营养型芽孢杆菌 生物防治 烟草 土传病原
Keywords:
Bacillus methylotrophicus biological control tobacco soilborne pathogeny
分类号:
S476
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.02.003
文献标志码:
A
摘要:
从番茄根际土壤分离到1株芽孢杆菌(Bacillus)FJSC01,其对烟草土传病原菌青枯拉尔氏菌(Ralstonia solanacearum)、胡萝卜软腐果胶杆菌(Pectobacterium carotovorum)、尖镰孢菌(Fusarium oxysporum)、胶孢炭疽菌(Colletotrichum gloeosporioides)、立枯丝核菌(Rhizoctonia solani)、疫霉菌(Phytophthora parasitica)和灰葡萄孢菌(Botrytis cinerea)等具有明显的拮抗作用.盆栽试验结果表明,菌株FJSC01对烟草青枯病的防效为40%.基于病原菌培养性状、形态特征、生理生化特性、16S rDNA系统演化分析,将菌株FJSC01鉴定为甲基营养型芽孢杆菌(Bacillus methylotrophicus).
Abstract:
Bacillus FJSC01 was isolated from the rhizosphere of tomato. It showed a strong antagonistic effect on tobacco soilborne pathogens, including bacteria such as Pectobacterium carotovorum and Ralstonia solanacearum, fungi such as Botrytis cinerea, Colletotrichum gloeosporioides, Fusarium oxysporum and Rhizoctonia solani, and oomycete of Phytophthora parasitica. Pot experiment showed that the bio-control efficacy of Bacillus FJSC01 against tobacco bacterial wilt disease caused by R.solanacearum reached 40%. Based on the culture traits, morphological characteristics, physiological and biochemical characteristics, and 16S rDNA gene phylogenetic analysis, Bacillus FJSC01 was identified as Bacillus methylotrophicus.

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备注/Memo

备注/Memo:
收稿日期:2020-05-25 修回日期:2020-07-16
基金项目:中国烟草总公司福建省公司科技项目(201735000024112).
作者简介:程敏(1984-),女.研究方向:植物病害生防菌.Email:2453315920@qq.com.通信作者刘国坤(1972-),男,教授.研究方向:植物病害生物防治.Email:liuguok@126.com.
更新日期/Last Update: 2021-02-15