[1]原晓龙,王 毅,杨文忠.青头菌(Russula virescens)聚酮合酶基因的克隆及碳源对该基因表达的影响[J].福建农林大学学报(自然科学版),2021,50(02):250-256.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.02.015]
　YUAN Xiaolong,WANG Yi,YANG Wenzhong.Cloning of a polyketide synthetase gene from Russula virescens and the effects of carbon sources on its expression[J].,2021,50(02):250-256.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.02.015]
点击复制

青头菌(Russula virescens)聚酮合酶基因的克隆及碳源对该基因表达的影响()
分享到：

福建农林大学学报（自然科学版）[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年02期
页码:
250-256
栏目:
生命科学
出版日期:
2021-02-15

文章信息/Info

Title:
Cloning of a polyketide synthetase gene from Russula virescens and the effects of carbon sources on its expression
文章编号:
1671-5470(2021)02-0250-07
作者:
原晓龙;  王 毅;  杨文忠
云南珍稀濒特森林植物保护和繁育重点实验室,云南 昆明 650201
Author(s):
YUAN Xiaolong;  WANG Yi;  YANG Wenzhong
Laboratory of Forest Plant Cultivation and Utilization/Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration, Yunnan Academy of Forestry and Grassland, Kunming, Yunnan, 650201, China
关键词:
青头菌;  聚酮合酶;  聚类分析;  基因表达
Keywords:
Russula virescens;  polyketide synthetase;  phylogenetic analysis;  gene expression
分类号:
Q939.95
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.02.015
文献标志码:
A
摘要:
为了解青头菌中具体的聚酮合酶基因,从青头菌(Russula virescens)基因组挖掘并克隆到一个聚酮合酶(polyketide synthase, PKS)基因(RvPKS),通过生物信息学分析预期其功能,并检测了不同浓度碳源添加物对该基因表达的影响.结果表明:RvPKS基因(GenBank登录号:MT655136)的cDNA长度为6039 bp,编码2 012个氨基酸,结构域组织顺序为SAT-KS-AT-ACP-TE,各结构域的保守活性氨基酸序列为SAT(GIIGFSSGII)、KS(DTACSSSL)、AT(NVVEAHGTGTQ)、ACP(VVGHSLGEYVA)和TE(LGGWSLGGIIA); 预测的RvPKS编码蛋白与火丝菌(Pyronema omphalodes)、鸡枞菌属(Termitomyces sp.)、白环菇属(Leucoagaricus sp.)、乌氏曲霉(Aspergillus udagawae)、丝曲霉(Aspergillus lentulus)、Penicillium subrubescens等相关聚酮合酶蛋白聚为一支,该蛋白可能催化合成一种新型的分生孢子色素.此外,2%山梨醇和10%蔗糖能强烈诱导RvPKS基因的表达.
Abstract:
In order to comprehend the specific polyketide synthetase gene of Russula virescens, a polyketide synthetase gene(RvPKS)was cloned from the genome of R.virescens by genome mining. The function of the gene was analyzed by bioinformatics. The expression of the RvPKS gene in R.virescens was investigated under different concentrations of carbon sources. The results showed that the cDNA of the RvPKS gene was 6 039 bp in full length, encoding 2 012 amino acids. Its conserved domain was SAT-KS-AT-ACP-TE in sequence, with its conserved active amino acid sites being SAT(GIIGFSSGII), KS(DTACSSSL), AT(NVVEAHGTGTQ), ACP(VVGHSLGEYVA)and TE(LGGWSLGGIIA), respectively. The proteins encoded by RvPKS were clustered with those from Pyronema omphalodes, Termitomyces sp, Leucoagaricus sp., Aspergillus udagawae, Aspergillus lentulus and Penicillium subrubescens, suggesting that RvPKS might catalyze a new type of conidial pigment. The expression of the RvPKS gene was intensively induced by 2% sorbitol and 10% sucrose.

参考文献/References:

[1] 黄萍,沈孝善.绿菇子实体菌株的原生质体分离及再生菌株的获得[J].西南农业学报,2001,14(1):91-95.
[2] 朱启顺,杨大智.绿菇与云南松共生关系的研究[J].中国食用菌,1999,18(5):19-20.
[3] 王勃,周文忠,董胜强,等.青头菌子实体发育进程中挥发性成分分析[J].食品与发酵工业,2014,40(8):178-183.
[4] SUN Z, ZHANG L, ZHANG B, et al. Structural characterisation and antioxidant properties of polysaccharides from the fruiting bodies of Russula virescens[J]. Food Chemistry, 2010,118(3):675-680.
[5] 马红英,杨明挚,张汉波,等.青头菌的分离纯化及ITS序列鉴定[J].中国食用菌,2013,32(3):41-43.
[6] LI GJ, ZHAO R L, ZHANG CL, et al. A preliminary DNA barcode selection for the genus Russula(Russulales, Basidiomycota)[J]. Mycology, 2019,10(2):61-74.
[7] TAE H, SOHNG J K, PARK K. MapsiDB: an integrated web database for type I polyketide synthases[J]. Bioprocess and Biosystems Engineering, 2009,32(6):723-727.
[8] 朱峰,乔建军.聚酮合成酶底物专一性的研究进展[J].中国抗生素杂志,2006,31(11):641-645,664.
[9] KUSUYA Y, TAKAHASHINAKAGUCHI A, TAKAHASHI H, et al. Draft genome sequence of the pathogenic filamentous fungus Aspergillus udagawae strain IFM46973T[J]. Genome Announcements, 2015,3(4).~ 10.1128/genomeA.00834-15.
[10] TRAEGER S, ALTEGOER F, FREITAG M, et al. The genome and development-dependent transcriptomes of Pyronema confluens: a window into fungal evolution[J]. PLOS Genetics, 2013,9(9).~ 10.1371/journal.pgen.1003820.
[11] 汤建国.变绿红菇化学成分研究[J].中草药,2008,39(12):1776-1778.
[12] 徐丹先,林佶,段志敏,等.云南野生青头菌的化学成分分析[J].现代预防医学,2012,39(21):37-38.
[13] 王婷婷,樊建,赵天瑞.青头菌汁液对香蕉果酒发酵的影响[J].中国食用菌,2012,31(2):54-56.
[14] 孙忠伟,张丽香,王愈,等.变绿红菇子实体多糖的理化性质及其抗肿瘤活性[J].农产品加工学刊,2009(7):77-81.
[15] MILLER S L, BUYCK B. Molecular phylogeny of the genus Russula in Europe with a comparison of modern infrageneric classifications[J]. Mycological Research, 2002,106(3):259-276.
[16] SHIMONO Y, KATO M, TAKAMATSU S. Molecular phylogeny of Russulaceae(Basidiomycetes; Russulales)inferred from the nucleotide sequences of nuclear large subunit Rdna[J]. Mycoscience, 2004,45(5):303-316.
[17] CRAWFORD J, DANCY B, HILL E, et al. Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase[J]. Proceedings of the National Academy of Ences of the United States of America, 2006,103(45):16728-16733.
[18] BUKELSKIS D, DABKEVICIENE D, LUKOSEVICIUTE L, et al. Screening and transcriptional analysis of polyketide synthases and non-ribosomal peptide synthetases in bacterial strains from Krubera-Voronja Cave[J]. Frontiers in Microbiology, 2019,10:2149. ~10.3389/fmicb.2019.02149.
[19] PARK H, MIN B, JANG Y, et al. Comprehensive genomic and transcriptomic analysis of polycyclic aromatic hydrocarbon degradation by a mycoremediation fungus, Dentipellis sp. KUC8613[J]. Applied Microbiology and Biotechnology, 2019,103(6):8145-8155.
[20] KOMAKI H, SAKURAI K, HOSOYAMA A, et al. Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Streptomyces strains[J]. Scientific Reports, 2018,8(1):6888. ~ 10.1038/s41598-018-24921-y.
[21] 才晓玲,何伟,安福全.青头菌研究进展[J].现代农业科技,2014(17):122-125.
[22] 马红英.云南常见食用菌分离、培养及鉴定[D].昆明:云南大学,2013.
[23] 王毅,周旭,许宰铣,等.长松萝中一个聚酮合酶基因簇的克隆和鉴定[J].微生物学报,2014,54(7):770-777.
[24] WANG W J, LI D Y, LI Y C, et al. Caryophyllene sesquiterpenes from the marine-derived Fungus Ascotricha sp. ZJ-M-5 by the one strain-many compounds strategy[J]. Journal of Natural Products, 2014,77(6):1367-1371.
[25] VALARMATHI R, HARIHARAN G N, VENKATARAMAN G, et al. Characterization of a non-reducing polyketide synthase gene from lichen Dirinaria applanata[J]. Phytochemistry, 2009,70(6):721-729.

备注/Memo

备注/Memo:
收稿日期:2020-05-13 修回日期:2020-06-29
基金项目:国家自然科学基金项目(31860177); 2019中央引导地方发展专项基金(云南松研究开发中心建设); 云南省林业和草原科学院创新基金项目(MS2019-09).
作者简介:原晓龙(1986-),男,助理研究员.研究方向:分子生物学.Email:xiaolony@126.com.通信作者王毅(1981-),男,副研究员.研究方向:分子生物学.Email:22825818@qq.com.
更新日期/Last Update: 2021-02-15