[1]王如梦,杜逸菲,高玢垣,等.依赖于JA信号的蛋白酶抑制剂介导番茄对斜纹夜蛾的抗性[J].福建农林大学学报(自然科学版),2021,50(05):595-600.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.05.003]
 WANG Rumeng,DU Yifei,GAO Binyuan,et al.JA-dependent protease inhibitor mediates resistance of tomato against Spodoptera litura[J].,2021,50(05):595-600.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.05.003]
点击复制

依赖于JA信号的蛋白酶抑制剂介导番茄对斜纹夜蛾的抗性()
分享到:

福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年05期
页码:
595-600
栏目:
出版日期:
2021-09-25

文章信息/Info

Title:
JA-dependent protease inhibitor mediates resistance of tomato against Spodoptera litura
文章编号:
1671-5470(2021)05-0595-06
作者:
王如梦1 杜逸菲1 高玢垣1 丁元鑫1 孙仲享12
(1.福建农林大学农学院,福建 福州 350002; 2.中山大学有害生物控制与资源利用 国家重点实验室,广东 广州 510275)
Author(s):
WANG Rumeng1 DU Yifei1 GAO Binyuan1 DING Yuanxin1 SUN Zhongxiang12
(1.College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2.State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, Guangdong 510275, China)
关键词:
茉莉酸 蛋白酶抑制剂 番茄 斜纹夜蛾
Keywords:
jasmonic acid protease inhibitor tomato Spodoptera litura
分类号:
S43
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.05.003
文献标志码:
A
摘要:
蛋白酶抑制剂(PI)是番茄防御虫害的重要次生代谢物,茉莉酸(JA)信号在植物抗虫中发挥重要作用.然而,PI和JA在番茄抗虫中的关系与功能尚不明确.以重要鳞翅目害虫斜纹夜蛾、野生型番茄CM、JA突变体spr8和系统素过表达35S∷PS为材料,研究PI和JA在番茄抗虫中的关系与功能.结果表明,与取食对照CM相比,取食35S∷PS的斜纹夜蛾体重增量显著下降,而取食spr8的斜纹夜蛾体重增量显著上升,表明JA信号和系统素都参与了番茄抗虫过程.虫害后CM与35S∷PS中JA合成基因lapA1和pin2表达量显著上调,而spr8中JA合成基因表达量无明显变化,表明番茄抗虫依赖于JA信号.虫害还可以诱导CM合成PI,且35S∷PS中PI含量显著高于CM,spr8中PI含量显著低于CM,说明系统素和JA信号在PI合成中起重要作用.当取食添加PI的饲料时,斜纹夜蛾体重增长率显著下降.以上结果表明番茄受到虫害后通过JA信号调控PI的合成,从而提高对斜纹夜蛾的抗性.开放科学(资源服务)
Abstract:
Proteinase inhibitor(PI)is an important secondary metabolite in tomato for defense against insect pests. Jasmonic acid(JA)signal plays an important role in plant resistance to pests. However, the relationship and function of PI and JA in tomato resistance to pests remain unclear. The important Lepidoptera pests Spodoptera litura, wild type tomato CM, JA mutant spr8 and systemin overexpression 35S∷PS were investigated. The results showed that compared with feeding on the control CM, the weight increment of the larvae feeding on 35S∷PS decreased significantly, while the weight increment of the larvae feeding on the spr8 group increased significantly, indicating that both JA signal and systemin were involved in tomato resistance and defense against pest. The expressions of the genes required for JA synthesis, lapA1 and pin2 increased significantly after the insect feeding on CM and 35S∷PS, while no significant changes were observed in the spr8 group, indicating that the resistance of tomato to pests depends on JA signal. Moreover, PI was synthesized in CM after being attacked by S.litura, and the content of PI was significantly higher in 35S∷PS than that in CM but significantly lower in spr8, indicating that systemin and JA signal played important roles in PI synthesis. When fed with PI-added feed, the weight gain rate of S.litura significantly decreased. The above results indicated that PI depends on JA signal to mediate tomato resistance to S.litura.

参考文献/References:

[1] 刘佰明,谷希树,徐维红,等.番茄抗虫机制及其转基因种质资源抗性鉴定概述[C]∥中国植物保护学会.植物保护与现代农业——中国植物保护学会2007年学术年会论文集.北京:中国植物保护学会,2008:423-428.
[2] 崔伟康,杨鹏,廉法卓,等.茉莉酸信号转导途径介导番茄对棉铃虫的抗性[J].福建农林大学学报(自然科学版),2016,45(4):371-375.
[3] 姚文辉.斜纹夜蛾的生物学特性[J].华东昆虫学报,2005,14(2):122-127.
[4] HUANG S J, XU J F, HAN Z J. 2006. Baseline toxicity data of insecticides against the common cutworm[J]. International Journal of Pest Management, 2006,52(3):209-213.
[5] 李玉玺,刘俊华,栾明,等.系统素、茉莉酸在番茄系统伤反应中的作用[J].广西植物,2009,29(4):541-547.
[6] 桂连友,刘树生,陈宗懋.外源茉莉酸和茉莉酸甲酯诱导植物抗虫作用及其机理[J].昆虫学报,2004,47(4):507-514.
[7] 闫留华.番茄Spr8基因调控植物对害虫免疫反应的机理研究[D].北京:中国科学院大学,2014.
[8] 程仲毅,薛庆中.植物蛋白酶抑制剂基因结构、调控及其控制害虫的策略[J].遗传学报,2003,30(8):790-796.
[9] 曹鑫,孙文婷,邹世慧,等.植物蛋白酶抑制剂抗虫基因工程研究现状与对策研究[J].南方农业,2011,5(5):4-10.
[10] RYAN C A. Proteinase inhibitors in plants, genes for improving defenses against insects and pathogens[J]. Annual Review of Phytopathology, 1990,28:425-449.
[11] RYAN C A. The search for the proteinase inhibitor-inducing factor, PIIF[J]. Plant Molecular Biology, 1992,19:123-133.
[12] RYAN C A. The systemin signaling pathway: differential activation of plant defensive genes[J]. Biochimica et Biophysica Acta, 2000,1477:112-121.
[13] YAN L H, ZHAI Q Z, WEI J N, et al. Role of tomato lipoxygenase D in wound-induced jasmonate biosynthesis and plant immunity to insect herbivores[J]. PLoS Genetics, 2017,9(12):e1003964.
[14] KAWAZU K, MOCHIZUKI A, SATO Y, et al. Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes[J]. Arthropod-Plant Interactions, 2012,6(2):221-230.
[15] OLSON B J S C, MARKWELL J. Assays for determination of protein concentration[J]. Current Protocols in Protein Science, 2007,48:1-29.
[16] MCGURL B, OROZCO-CARDENAS M, PEARCE G, et al. Over expression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis[J]. Proceedings of the National Academy of Sciences, 1994,91:9799-9802.
[17] 刘丽艳.番茄(Lycopersion esculutum)抗虫缺失突变体defl的鉴定及相应基因的定位[D].哈尔滨:东北农业大学,2004.
[18] 李常保,孙加强,蒋红玲,等.番茄系统抗性反应的信号转导[J].科学通报,2005,50(16):1677-1683.
[19] TIAN D L, PEIFFER M, DEMORAES C M, et al. Roles of ethylene and jasmonic acid in systemic induced defense in tomato(Solanum lycopersicum)against Helicoverpa zea[J]. Planta, 2014,239:577-589.
[20] 吴国昭,朱克岩,曾任森.大豆胰蛋白酶抑制剂对斜纹夜蛾生长发育的影响[J].生态环境学报,2013,22(8):1335-1340.
[21] KARBAN R, BALDWIN I T. Induced responses to herbivory[M]. Chicago: University of Chicago Press, 2007.
[22] HOWE G A, JANDER G. Plant immunity to insect herbivores[J]. Annual Review of Plant Biology, 2008,59(1):41-66.
[23] 浦冠勤,殷培峰,曲晓华.植物蛋白酶抑制剂抗虫作用的研究进展[C]∥中国蚕学会.中国蚕学会第五次全国桑保学术研讨会论文集.镇江:中国蚕学会,2004:268-274.

备注/Memo

备注/Memo:
收稿日期:2020-10-13 修回日期:2020-11-10 基金项目:福建省自然科学基金(2020J01530); 福建农林大学杰出青年科研人才计划项目(KXJQ19009); 中山大学有害生物控制与资源利用国家重点实验室开放课题(2020SKLBC-KF04); 福建农林大学科技创新专项基金项目(CXZX2020005A). 作者简介:王如梦(1995-),女.研究方向:作物抗性.Email:1844617645.qq.com.通信作者孙仲享(1986-),男,讲师,硕士生导师.研究方向:植物-昆虫互作.Email:szx@fafu.edu.cn.
更新日期/Last Update: 1900-01-01