[1]苏奇倩,李 林,丁豪杰,等.苹果果色转录因子MYB110基因多态性分析[J].福建农林大学学报(自然科学版),2021,50(05):588-594.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.05.002]
 SU Qiqian,LI Lin,DING Haojie,et al.Polymorphism analysis of fruit-color related transcription factor MYB110 gene in apples[J].,2021,50(05):588-594.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.05.002]
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苹果果色转录因子MYB110基因多态性分析()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年05期
页码:
588-594
栏目:
出版日期:
2021-09-25

文章信息/Info

Title:
Polymorphism analysis of fruit-color related transcription factor MYB110 gene in apples
文章编号:
1671-5470(2021)05-0588-07
作者:
苏奇倩12 李 林12 丁豪杰12 李晓锋12 徐其静12 RENSING Christopher13 刘 雪12
(1.西南林业大学环境修复与健康研究院,云南 昆明 650224; 2.西南林业大学生态与环境学院, 云南 昆明 650224; 3.福建农林大学资源与环境学院,福建 福州 350002)
Author(s):
SU Qiqian12 LI Lin12 DING Haojie12 LI Xiaofeng12 XU Qijing12 RENSING Christopher13 LIU Xue12
(1.Institute of Environment Remediation and Health, Southwest Forestry University, Kunming, Yunnan 650224, China; 2.Institute of Ecology and Environment, Southwest Forestry University, Kunming, Yunnan 650224, China; 3.Institute of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China )
关键词:
苹果果色 花青素 转录因子 MYB110 多态性
Keywords:
apple fruit color anthocyanin transcription factor MYB110 polymorphism
分类号:
S666.1
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.05.002
文献标志码:
A
摘要:
苹果果色主要由花青素等色素控制,花青素是植物的一类黄酮类次生代谢物,赋予植物各种颜色,转录因子MYB110基因参与植物花青素合成调控.本研究通过对MYB110转录因子扩增测序,检测MYB110在22种苹果属植物中的多态性.结果表明,MYB110基因编码区长度均为602 bp,扩增出新疆野苹果和西府海棠等7种材料的基因序列,其多态性位点共有52个,翻译成蛋白质后多态性位点为28个,且多态性位点主要位于非功能结构域,不同苹果果色间存在基因调控差异.
Abstract:
The fruit color of apple is mainly controlled by anthocyanins, which is a class of flavonoid secondary metabolite in plants that render them various colors. Transcription factor MYB110 is involved in the regulation of plant anthocyanin synthesis. In this study, the polymorphism of the MYB110 gene in 22 species of Malus was detected by sequencing. The MYB110 gene was successfully amplified from 7 Malus species, including M.sieversii, M.micromalus, and etc. The sequencing results showed that the MYB110 gene encoding region was 602 bp. Polymorphism analysis of the MYB110 gene revealed that there were 52 and 28 polymorphic sites in the DNA and protein sequences, respectively, which mainly existed in the non-functional domains. This indicated that the regulation of anthocyanin synthesis by MYB110 is different among different apple fruit colors.

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备注/Memo

备注/Memo:
收稿日期:2021-01-08 修回日期:2021-04-05 基金项目:国家自然科学基金项目(41867066、41907129); 云南省自然科学基金项目(2019FB032); 云南省教育厅科学研究基金项目(2020Y0391、2020J0406); 云南省高端外国专家项目(YNQR-GDWG-2018-017). 作者简介:苏奇倩(1997-),女.研究方向:土壤污染与修复.Email:qiqian_su@163.com.通信作者刘雪(1987-),女,副研究员,硕士生导师.研究方向:环境污染与食品安全.Email:liuxue20088002@126.com.
更新日期/Last Update: 1900-01-01