[1]黄在兴,宋昭昭,梅 兰,等.基于转录组学的巨菌草内生链霉菌拮抗玉蜀黍平脐蠕孢的分子机理[J].福建农林大学学报(自然科学版),2021,50(05):686-693.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.05.016]
 HUANG Zaixing,SONG Zhaozhao,MEI Lan,et al.Molecular mechanism of antagonistic effect of endophytic Streptomyces sp. of Pennisetum giganteum z.x.lin on Bipolaris maydis based on transcriptome[J].,2021,50(05):686-693.[doi:10.13323/j.cnki.j.fafu(nat.sci.).2021.05.016]
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基于转录组学的巨菌草内生链霉菌拮抗玉蜀黍平脐蠕孢的分子机理()
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福建农林大学学报(自然科学版)[ISSN:1671-5470/CN:35-1255/S]

卷:
50卷
期数:
2021年05期
页码:
686-693
栏目:
出版日期:
2021-09-25

文章信息/Info

Title:
Molecular mechanism of antagonistic effect of endophytic Streptomyces sp. of Pennisetum giganteum z.x.lin on Bipolaris maydis based on transcriptome
文章编号:
1671-5470(2021)05-0686-08
作者:
黄在兴1 宋昭昭1 梅 兰1 李 曼1 刘朋虎1 苏德伟1 林占熺1 鲁国东12
(1.福建农林大学国家菌草工程技术研究中心; 2.福建农林大学植物保护学院,福建 福州 350002)
Author(s):
HUANG Zaixing1 SONG Zhaozhao1 MEI Lan1 LI Man1 LIU Penghu1 SU Dewei1 LIN Zhanxi1 LU Guodong12
(1.National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University; 2.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)
关键词:
生物防治 RNA-seq 高通量测序 差异基因
Keywords:
biological control RNA-sequencing high-throughput sequencing differential gene
分类号:
S154.4
DOI:
10.13323/j.cnki.j.fafu(nat.sci.).2021.05.016
文献标志码:
A
摘要:
通过Illumina Hiseq4000高通量测序平台,采用PE150测序策略研究了玉蜀黍平脐蠕孢在链霉菌发酵液胁迫下(S组)的转录组变化,共得到128 613 786个clean reads,筛选出4 559个差异基因.与无发酵液的对照组相比,S组中有2 283个基因表达上调,2 276个基因表达下调; 从中挑选出表达量差异最大的22个基因作为内生链霉菌调控拮抗的拟关键基因,这些基因多涉及氨基酸合成和代谢以及信号转导途径,说明链霉菌发酵液对玉蜀黍平脐蠕孢的氨基酸生物合成和MAPK 信号通路相关基因的影响显著; 采用实时荧光定量PCR验证其中5个基因的表达量,其趋势与转录组分析结果基本一致.开放科学(资源服务)
Abstract:
Transcriptome changes of Bipolaris maydis under the stress of Streptomyces sp. fermentation broth were evaluated using PE150 sequencing strategy under Illumina Hiseq4000 high-throughput sequencing platform. A total of 128 613 786 clean reads were obtained, and 4 559 differential genes were screened. Compared with the CK group, 2 283 genes were up-regulated and 2 276 genes were down-regulated. Then 22 genes, witnessing the most changes were designated as the putative key genes in playing antagonist roles against B.maydis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that most of the genes were involved in amino acid synthesis and metabolism, and signal transduction pathways, suggesting that the fermentation broth of Streptomyces sp. had significant effects on the genes related to amino acid biosynthesis and MAPK signaling pathway in B.maydis. Meanwhile real-time fluorescent quantitative PCR was conducted to verify the expressions of the 5 genes, and their expression trends were consistent with the results of transcriptome analysis.

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备注/Memo

备注/Memo:
收稿日期:2020-09-25 修回日期:2020-10-20 基金项目:中央引导地方科技发展专项(2018L3003). 作者简介:黄在兴(1995-),男.研究方向:基因工程.Email:1227159839@qq.com.通信作者鲁国东(1967-),男,教授,博士生导师.研究方向:植物与病原菌相互作用的分子机制.Email:lgd@fafu.edu.cn.
更新日期/Last Update: 1900-01-01